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Single stranded substrate DNA (SS) and enzyme chain DNA (ES) were hybridized in pH 7.0 HEPES(7365-45-9) buffer solution

Single stranded substrate DNA (SS) and enzyme chain DNA (ES) were hybridized at 80 °C to form double-stranded DNA (dsDNA) in the presence of 0.19 mol·L-1 NaCl in pH 7.0 HEPES(7365-45-9) buffer solution. Cu2 + cleavable dsDNA in the dsDNA in the release of single-stranded DNA (ssDNA), the ssDNA and gold nanoparticles (NG) to form NGssDNA conjugate is not NaC1 aggregation, and unprotected NG aggregation to form a larger particle size aggregation (NGA), at 627nm there is a strong resonance rayleigh scattering peak. With the increase of Cu2 + concentration, the resonance rayleigh scattering peak decreases, and its decreasing value △ I and Cu2 + concentration in the range of 15 ~ 1250 nmol·L-1 linear relationship, the regression equation is △ I = 0.17c-2.3, the linear correlation coefficient was 0.9895, and the detection limit was 8 nmol·L-1. Based on this, a resonant Rayleigh scattering spectroscopy method with high sensitivity, high selectivity and simple determination of Cu2 + was established. The method is used for the detection of Cu2 + in water samples.

 

 

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