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Tris(77-86-1) Production Method and Usage

Tris(77-86-1) Production Method:
The Tris(77-86-1) was dissolved in a small amount of double distilled water (300-500 ml). After adding HCl, the pH was adjusted to 7.6 with HCl (1N) or NaOH (1N), and finally distilled twice to 1000 ml. This solution is stock solution, 4 ℃ refrigerator to save.
Note: Tris-Hcl PH value changes with the temperature changes, it should wait until reach room temperature and then test, so that the measured results are more reliable.
Use:
Tris(77-86-1) buffers are not only widely used as solvents for nucleic acids and proteins, but also for many important uses. Tris is used for protein crystal growth at different pH conditions. The low ionic strength of the Tris buffer can be used for the formation of intermediate fibers of the C. elegans laminus. Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is the preparation of surfactants, vulcanization accelerators and intermediates of some drugs. Tris is also used as a titration standard.
Buffer characteristics:
Tris(77-86-1) is weak base, and its pKa is 8.1 at room temperature (25 °C). According to the buffer theory, the effective buffer of Tris buffer is between pH 7.0 and 9.2.
The pH of the aqueous solution of Tris base is about 10.5, and hydrochloric acid is usually added to adjust the pH to the desired value to obtain a buffer of this pH. But should also pay attention to the effect of temperature on Tris's pKa.
Tris(77-86-1) is a biological buffer; used for gel electrophoresis configuration buffer. As an alkaline drug, for acidosis correction, and will not cause increased carbon dioxide retention. Each 7.28% THAM 2-3ml /kg (isotonic solution of 3.64%), with the same amount of 5% -10% glucose solution diluted slowly after the drop. Clinical abbreviation for the THAM.

 

 

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