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Effect of guanidine hydrochloride(50-01-1) concentration on refolding yield

In this paper, the refolding effect of the artificial chaperone system (β-cyclodextrin-β-CD and detergent cetyltrimethylammonium bromide-CTAB) was studied in this paper. And the effects of the concentration of guanidine hydrochloride(50-01-1) on the refolding yield were compared. The results showed that under the same refolding condition, the refolding of the denatured protein could be improved by the flowwise operation Yield and the presence of artificial companion at this time will further improve the efficiency of refolding to the concentration of 1mg / mL denatured lysozyme final refolding yield of 87%; in the flow of operating conditions, the best guanidine hydrochloride concentration ( 1mol / L) to achieve the highest rate of refolding; at the same time in the case of the same refolding yield, the use of flow operation can be appropriate to reduce the concentration of guanidine hydrochloride(50-01-1) in the refolding solution 



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