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Effect of additive concentration on the rate of refolding at lower concentration of guanidine hydrochloride(50-01-1)

The effects of amide on the renaturation of denatured bovine carbonic acid dehydratase (CAB) were studied. The effects of the concentration of additive on the refolding yield at the lower concentration of guanidine hydrochloride(50-01-1) were investigated. 

The results showed that all kinds of additives containing amide groups could effectively promote CAB refolding. The amide compounds were similar to those of guanidine hydrochloride(50-01-1), that is, the refolding rate constant kN values were Which is higher than that of the additive, and decreases with the increase of the concentration of the additive. However, the aggregation rate constant kA has the minimum value at the optimum additive concentration, so that the kN / kA value is the largest and the refolding yield is the largest.



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